Identifying Genes Associated with Abiotic Stress Tolerance Suitable for CRISPR/Cas9 Editing in Upland Rice Cultivars Adapted to Acid Soils

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dc.audienceInvestigadorspa
dc.audience.contentCientíficospa
dc.contributor.authorBarrero, Luz Stella
dc.contributor.authorWillmann, Matthew
dc.contributor.authorCraft, Eric
dc.contributor.authorManna Akther, Kazi
dc.contributor.authorHarrington, Sandra E.
dc.contributor.authorHarrington, Sandra E.
dc.contributor.authorGlahn, Raymond
dc.contributor.authorPiñeros, Miguel
dc.contributor.authorMccouch, Susan
dc.coverage.countryColombiaspa
dc.date.accessioned2025-02-12T17:31:13Z
dc.date.available2025-02-12T17:31:13Z
dc.date.created2021-09
dc.date.issued2021
dc.description.abstractFive genes of large phenotypic effect known to confer abiotic stress tolerance in rice were selected to characterize allelic variation in commercial Colombian tropical japonica upland rice cultivars adapted to drought-prone acid soil environments (cv. Llanura11 and Porvenir12). Allelic variants of the genes ART1, DRO1, SUB1A, PSTOL1, and SPDT were characterized by PCR and/or Sanger sequencing in the two upland cultivars and compared with the Nipponbare and other reference genomes. Two genes were identified as possible targets for genome editing: SUB1A, Submergence 1A, to improve tolerance to flooding, and SPDT (SULTR3;4), SULTR-like Phosphorus Distribution Transporter, to improve phosphorus utilization efficiency and grain quality. Based on technical and regulatory considerations, SPDT was targeted for editing. The two upland cultivars were shown to carry the SPDT wild-type (non-desirable) allele based on sequencing, RNA expression, and phenotypic evaluations under hydroponic and greenhouse conditions. A gene deletion was designed using the CRISPR/Cas9 system and specialized reagents and protocols were developed for SPDT editing, including vectors targeting the gene, protoplast transfection transient assays, and plant regeneration and transformation protocols by Agrobacterium and ribonucleoprotein complexes. The desired edits were confirmed in protoplasts and serve as the basis for ongoing plant transformation experiments aiming to improve the P-use efficiency of upland rice grown in acidic soils.spa
dc.description.productionsystemsArrozspa
dc.description.scientificnameOryza sativa
dc.format.mimetypeapplication/pdf
dc.identifierhttps://www.researchsquare.com/article/rs-864595/v1
dc.identifier.doihttps://doi.org/10.21203/rs.3.rs-864595/v1
dc.identifier.instnameinstname:Corporación colombiana de investigación agropecuaria AGROSAVIAspa
dc.identifier.reponamereponame:Biblioteca Digital Agropecuaria de Colombiaspa
dc.identifier.urihttp://hdl.handle.net/20.500.12324/40673
dc.language.isoeng
dc.publisherResearcherspa
dc.publisher.placeBogotá (Colombia)spa
dc.relation.citationendpage19
dc.relation.citationissue1
dc.relation.citationstartpage1
dc.relation.citationvolume1
dc.relation.ispartofjournalResearch Squarespa
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dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/
dc.sourceResearch Square; (2021): Research Square (Sep.);p. 1-19.spa
dc.subject.agrovocOryza sativaspa
dc.subject.agrovocGenspa
dc.subject.agrovocEstrés abióticospa
dc.subject.agrovocSuelo ácidospa
dc.subject.agrovocurihttp://aims.fao.org/aos/agrovoc/c_5438
dc.subject.agrovocurihttp://aims.fao.org/aos/agrovoc/c_3214
dc.subject.agrovocurihttp://aims.fao.org/aos/agrovoc/c_35768
dc.subject.agrovocurihttp://aims.fao.org/aos/agrovoc/c_89
dc.subject.faoCultivo - F01spa
dc.subject.redTransitoriosspa
dc.titleIdentifying Genes Associated with Abiotic Stress Tolerance Suitable for CRISPR/Cas9 Editing in Upland Rice Cultivars Adapted to Acid Soilsspa
dc.title.translatedIdentifying Genes Associated with Abiotic Stress Tolerance Suitable for CRISPR/Cas9 Editing in Upland Rice Cultivars Adapted to Acid Soilsspa
dc.type.coarhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.type.driverinfo:eu-repo/semantics/article
dc.type.localArtículo científicospa
dc.type.localengarticleeng
dc.type.redcolhttps://purl.org/redcol/resource_type/ART
dc.type.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85

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